CellStor® Freezing Bags

The Cryopreservation Bags are used for the storage of Blood & Blood Components, Cell & Cellular Components and Tissues

Efficient Design – Low dead volumes, high recoveries
Closed System
Compatible with most standard racking systems
Custom Designs Available
Gamma Sterile, Single-use
In-built bag label area for certain bags
Tested for non-toxic, and endotoxin
Inert and highly bio-compatible
DMSO/DMSO-Dextran Compatible

CellStor Freezing Bags are suitable for cryopreserving biological samples down to -196°C in both the liquid and vapour phases.

CellStor freezing bags are used to freeze and cryopreserve cells (primary cells and cell lines), and cellular components, blood and blood components, tissues, and other biological products such as vaccines, proteins, exosomes, etc. for use in various treatment modalities such as stem cell therapy, CAR-T cell therapy, NK cell therapy, gene therapy, tissue engineering, and regenerative medicine.

CellStor freezing bags are made up of special grade Ethylene-vinyl acetate (EVA), also known as polyolefin. They retain their flexibility even at freezing temperatures and have high puncture resistance.

CellStor freezing bags are resistant to gas and liquid permeation, they facilitate cross contamination-free storage and culture.

CellBios is the first company in the World to offer freezing bags with 50+ different fill sizes and configurations for various applications. Each Freezing bag is available with 10 different port configurations with or without Overwrap bags providing the end user ultimate flexibility in choosing a bag that is most suitable for their application. Further options are available via our custom order process through which we can accommodate most end users’ process needs.

Ranging from 1 ml to 3 Liters

Features

Safe. Economical.

The CellStor freezing bag set is a safe, economical way to process and preserve blood & blood components, cell & cellular components, tissues, and other biologics.

Low Temperature Durability

The freezing bags are made from special Ethylene Vinyl Acetate (EVA)/Polyolefin film, which combines tough, low temperature durability with excellent transparency, flexibility, impact and puncture resistance.

Reduce Contamination

The bag film has good vapour and gas barrier properties, helping to minimize sample cross contamination.

Long Term Storage

The freezing bag is designed to tolerate long term storage at temperature down to -196ºC, the optimal storage condition of cells, tissue, organs and other biologics.

Retains Biological Specimens

The film material is biologically inert which will ensure that the stored biologics are unaltered by their environment.

The closed system bags ensures microbial free environment to the product

Advantages

CellStor bags are pre-assembled, single-use, gamma sterilized, closed systems
Specially designed for controlled freezing, thawing, and long-term cryostorage
Improved design with reduced dead spaces to ensure proper mixing of the sample, forming a homogenous mixture preventing cell death/loss during cryopreservation.
Smooth inner surface of CellStor bag doesn’t trap the cells and helps in maximizing the recovery of thawed product.
Extra-long EVA tubing enables easy sampling for quality control testing.
Needle free connection options help reduce the risk of contamination and leakage. This also ensures a closed system even after disconnecting the syringe.
Hanger holes are provided for easy handling of the bags.
Labelling space is provided for recording sample information.
Two female luers and one male luer are provided as the default port configuration. However, the port configuration can be customized as per process requirements.
Freezing bags are available in 50+ different sizes (from 1 ml to 3 Liters) offering flexibility for use in various applications.
CellStor freezing bags are available with single, dual, and multi-compartment, top and bottom tubes and ports.
Multi compartment bags are designed to facilitate easy sample distribution into various smaller bags (for different QC studies) and larger storage bags in a single filling operation. This reduces the need for multiple manual welds and seals, reducing contamination risk.
Our Freezing bags are individually packed in Tyvek pouches to facilitate aseptic processing.

Bag Validation: THP-1 and CD34+ cells were suspended in 8% dimethyl sulfoxide (DMSO) and frozen in CellStor freezing bags. Cells were thawed and counted, the average recovery of THP-1 and CD34+ cells were found to be 92% and 90% respectively (study acceptance criteria for thaw recovery was ≥ 70%).

Technical Specification

Description	Freezing Bag
Bag Dimensions	Refer “Specifications”
Bag Volumes	1 mL to 3000 mL
Materials of Construction	Freezing Bag, Donor Tubing: Ethylene Vinyl Acetate (EVA) Extension Tubing: Polyvinyl Chloride (PVC)
Packaging	Individually packaged in a Tyvek pouch, 24 /box
Port Configuration	Straight Barb / Y-Barb (1), Needle Free Y (1), Male Luer with Cap (1), Female Luer with Cap (2), 4-Way PVC Y (1), Roller Clamps (3)
Shelf Life	3 Years
Sterilization	Gamma
Traceability	Lot number
Tubing	Donor Tubing (EVA) : ID 3 mm, OD 4.1 mm Length: 50 to 300 mm PVC Extension Tubing: ID 3 mm, OD 4.1 mm Length: 50 mm to 120 mm

Applications

CellStor Freezing Bags for Cell / Gene / Tissue Therapy and Bioprocess Applications

CellStor Freezing Bags are used for storing/ cryopreserving biological products such as cells, tissues, organelles, and their byproducts used in various healthcare applications and basic research.

CellStor bags are pre-assembled, single-use, gamma sterilized, closed systems that facilitate ideal freezing and thawing processes for cell/ gene/tissue therapy and bioprocess applications. They are specially designed for controlled freezing, thawing, and long-term cryo storage.

CellStor freezing bags are available in various sizes, ranging from 1 ml to 3 Liters (50+ different sizes) with single, dual, and multi-compartment, top and bottom tubes and ports.

Bags are designed to facilitate easy sample distribution into various smaller bags (for different QC studies) and larger storage bags in a single filling operation. This reduces the need for multiple manual welds and seals, reducing contamination risk while improving ease of use.

Specifications

Single Compartment

Part Number	Description	Dimension (W x L)	Nominal Volume	Recommended Freeze Volume
CSEAV01-01	CellStor^® Freezing Bag, 1 mL (Top & Bottom Tube/Port), S Assembly NF	21 x 31 mm	1 mL	500 μL - 1 mL
CSEVA02-01	CellStor^® Freezing Bag, 2 mL (Top & Bottom Tube/Port), S Assembly NF	26 x 36 mm	2 mL	1 mL - 2 mL
CSEVA03-01	CellStor^® Freezing Bag, 3 mL (Top & Bottom Tube/Port), S Assembly NF	26 x 44 mm	3 mL	1 mL - 3 mL
CSEVA05-01	CellStor^® Freezing Bag, 5 mL, S Assembly NF	26 x 67 mm	5 mL	2 mL - 5 mL
CSEVA05-02	CellStor^® Freezing Bag, 5 mL (Top & Bottom Tube/Port), S Assembly NF	26 x 67 mm	5 mL	2 mL - 5 mL
CSEVA06-01	CellStor^® Freezing Bag, 6 mL (Top & Bottom Tube/Port), S Assembly NF	26 x 76 mm	6 mL	2 mL - 6 mL
CSEVA07-01	CellStor^® Freezing Bag, 7 mL (Top & Bottom Tube/Port), S Assembly NF	38 x 62 mm	7 mL	3 mL - 7 mL
CSEVA10-01	CellStor^® Freezing Bag, 10 mL, S Assembly NF	38 x 67 mm	10 mL	5 mL - 10 mL
CSEVA10-02	CellStor^® Freezing Bag, 10 mL (Top & Bottom Tube/Port), S Assembly NF	38 x 79 mm	10 mL	5 mL - 10 mL
CSEVA12-01	CellStor^® Freezing Bag, 12 mL (Top & Bottom Tube/Port), S Assembly NF	48 x 76 mm	12 mL	5 mL - 12 mL
CSEVA15-01	CellStor^® Freezing Bag, 15 mL (Top & Bottom Tube/Port), S Assembly NF	44 x 99 mm	15 mL	6 mL - 15 mL
CSEVA20-01	CellStor^® Freezing Bag, 20 mL, S Assembly NF	71 x 67 mm	20 mL	10 mL - 20 mL
CSEVA20-02	CellStor^® Freezing Bag, 20 mL (Top & Bottom Tube/Port), S Assembly NF	44 x 122 mm	20 mL	10 mL - 20 mL
CSEVA25-02	CellStor^® Freezing Bag, 25 mL, S Assembly NF	88 x 67 mm	25 mL	10 mL - 25 mL
CSEVA25-03	CellStor^® Freezing Bag, 25 ml (Top & Bottom Tube/Port), S Assembly NF	54 x 123 mm	25 mL	10 mL - 25 mL
CSEVA30-02	CellStor^® Freezing Bag, 30 mL, S Assembly NF	105 x 67 mm	30 mL	10 mL- 30 mL
CSEVA30-03	CellStor^® Freezing Bag, 30 mL (Top & Bottom Tube/Port), S Assembly NF	66 x 123 mm	30 mL	10 mL - 30 mL
CSEVA50-01	CellStor^® Freezing Bag, 50 mL, S Assembly NF	76 x 194.5 mm	50 mL	10 mL - 30 mL
CSEVA50-02	CellStor^® Freezing Bag, 50 mL (Top & Bottom Tube/Port), S Assembly NF	66 x 144 mm	50 mL	10 mL - 30 mL
CSEVA150-01	CellStor^® Freezing Bag, 150 mL, S Assembly NF	126 x 144 mm	150 mL	25 mL - 60 mL
CSEVA250-01	CellStor^® Freezing Bag, 250 mL, S Assembly NF	126 x 155 mm	250 mL	30 mL - 80 mL
CSEVA500-01	CellStor^® Freezing Bag, 500 mL, S Assembly NF	126 x 210 mm	500 mL	50 mL - 120 mL
CSEVA750-01	CellStor^® Freezing Bag, 750 mL, S Assembly NF	126 x 241 mm	750 mL	80 mL - 190 mL
CSEVA1000-01	CellStor^® Freezing Bag, 1000 mL, S Assembly NF	173 x 323 mm	1000 mL	100 mL - 320 mL
CSEVA2000-01	CellStor^® Freezing Bag, 2000 mL, S Assembly NF	225 x 367 mm	2000 mL	200 mL - 540 mL
CSEVA3000-01	CellStor^® Freezing Bag, 3000 mL, S Assembly NF	270 x 434 mm	3000 mL	500 mL - 850 mL

Double Compartments

Part Number	Description	Dimension (W x L)	Nominal Volume	Recommended Freeze Volume
CSEVA2X5-01	CellStor^® Freezing Bag, 2x5 mL, S Assembly NF	52 x 67 mm	10 mL	4 mL - 10 mL
CSEVA2X5-02	CellStor^® Freezing Bag, 2x5 mL (Top & Bottom Tube/Port), S Assembly NF	52 x 67 mm	10 mL	4 mL - 10 mL
CSEVA2X10-01	CellStor^® Freezing Bag, 2x10 mL, S Assembly NF	76 x 67 mm	20 mL	10 mL - 20 mL
CSEVA2X10-02	CellStor^® Freezing Bag, 2x10 mL (Top & Bottom Tube/Port), S Assembly NF	76 x 79 mm	20 mL	10 mL - 20 mL
CSEVA2X12.5-01	CellStor^® Freezing Bag, 25 mL (Double Compartment), S Assembly NF	90 x 67 mm	25 mL	10 mL - 25 mL
CSEVA25-01	CellStor^® Freezing Bag, 25 mL (Double Compartment), S Assembly NF	90 x 67 mm	25 mL	10 mL - 25 mL
CSEVA30-01	CellStor^® Freezing Bag, 30 mL (Double Compartment), S Assembly NF	109 x 67 mm	30 mL	10 mL - 30 mL

Multi Compartments

Part Number	Description	Dimension (W x L)	Nominal Volume	Recommended Freeze Volume
CSEVA5X1-01	CellStor^® Freezing Bag, 5x1 mL (Top & Bottom Tube/ Port), S Assembly NF	105 x 31 mm	5 mL	2.5 mL - 5 mL
CSEVA5X2-01	CellStor^® Freezing Bag, 5x2 mL (Top & Bottom Tube/ Port), S Assembly NF	130 x 36 mm	10 mL	5 mL - 10 mL
CSEVA4X3-01	CellStor^® Freezing Bag, 4x3 mL (Top & Bottom Tube/ Port), S Assembly NF	105 x 44 mm	12 mL	4 mL - 12 mL
CSEVA5X3-01	CellStor^® Freezing Bag, 5x3 mL (Top & Bottom Tube/ Port), S Assembly NF	130 x 44 mm	15 mL	5 mL - 15 mL
CSEVA3X5-01	CellStor^® Freezing Bag, 3x5 mL, S Assembly NF	78 x 67 mm	15 mL	6 mL - 15 mL
CSEVA3X5-02	CellStor^® Freezing Bag, 3x5 mL (Top & Bottom Tube/ Port), S Assembly NF	78 x 67 mm	15 mL	6 mL - 15 mL
CSEVA4X5-01	CellStor^® Freezing Bag, 4x5 mL, S Assembly NF	105 x 67 mm	20 mL	8 mL - 20 mL
CSEVA4X5-02	CellStor^® Freezing Bag, 4x5 mL (Top & Bottom Tube/ Port), S Assembly NF	105 x 67 mm	20 mL	8 mL - 20 mL
CSEVA4X5-03	CellStor^® Freezing Bag, 4x5 mL (Single Tube), S Assembly NF	105x67 mm	20 mL	8 mL - 20 mL
CSEVA5X5-01	CellStor^® Freezing Bag, 5x5 mL, S Assembly NF	131 x 67 mm	25 mL	10 mL - 25 mL
CSEVA5X5-02	Cellstor^® Freezing Bag, 5x5 mL (Top & Bottom Tube/ Port), S Assembly NF	131 x 67 mm	25 mL	10 mL - 25 mL
CSEVA5X5-03	Cellstor^® Freezing Bag, 5x5 mL (Single Tube), S Assembly NF	131 x 67 mm	25 mL	10 mL - 25 mL
CSEVA4X6-01	CellStor^® Freezing Bag, 4x6 mL (Top & Bottom Tube/ Port), S Assembly NF	105 x 76 mm	24 mL	8 mL - 24 mL
CSEVA5X6-01	CellStor^® Freezing Bag, 5x6 mL (Top & Bottom Tube/ Port), S Assembly NF	130 x 76 mm	30 mL	10 mL - 30 mL
CSEVA3X10-01	CellStor^® Freezing Bag, 3x10 mL, S Assembly NF	114 x 67 mm	30 mL	15 mL - 30 mL
CSEVA3X10-02	CellStor^® Freezing Bag, 3x10 mL (Top & Bottom Tube/ Port), S Assembly NF	114 x 79 mm	30 mL	15 mL - 30 mL
CSEVA4X10-01	CellStor^® Freezing Bag, 4x10 mL, S Assembly NF	152 x 67 mm	40 mL	20 mL - 40 mL
CSEVA4X10-02	CellStor^® Freezing Bag, 4x10 mL (Top & Bottom Tube/ Port), S Assembly NF	152 x 79 mm	40 mL	20 mL - 40 mL

Lay Flat Freezing Bags

Part No.	Description	Dimension (W x L)	Nominal Volume	Recommended Freeze Volume
CSEVALF50-01	CellStor^® Lay Flat Freezing Bag, 50 mL, S Assembly NF	120 x 123 mm	50 mL	10-30 mL
CSEVALF150-01	CellStor^® Lay Flat Freezing Bag, 150 mL, S Assembly NF	120 x 144 mm	150 mL	25-60 mL
CSEVALF250-01	CellStor^® Lay Flat Freezing Bag, 250 mL, S Assembly NF	120 x 155 mm	250 mL	30-80 mL
CSEVALF500-01	CellStor^® Lay Flat Freezing Bag, 500 mL, S Assembly NF	120 x 210 mm	500 mL	50-120 mL
CSEVALF750-01	CellStor^® Lay Flat Freezing Bag, 750 mL, S Assembly NF	120 x 241 mm	750 mL	80-190 mL

Ordering Information - CellStor® Freezing Bags Ordering Information - CellStor® Lay Flat Freezing Bags

Stem Cell Therapy

Tissue and Organ Storage

Blood and Blood Component Storage

Reproductive Organ Preservation

Biotechnology

Microbial Technology

CellStor® Freezing Bags

The Cryopreservation Bags are used for the storage of Blood & Blood Components, Cell & Cellular Components and Tissues

Single Compartment

Dual Compartment

Multi Compartment